Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals

Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals

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Abstract MicroRNAs (miRNAs) are essential regulators involved in multiple biological processes.To achieve their gene repression groth cabernet sauvignon 2017 function, they are loaded in miRNA-specific Argonautes to form the miRNA-induced silencing complex (miRISC).Mammals and C.

elegans possess more than one paralog of miRNA-specific Argonautes, but the dynamic between them remains unclear.Here, we report the conserved dipeptidyl peptidase DPF-3 as an interactor of the miRNA-specific Argonaute ALG-1 in C.elegans.

Knockout of dpf-3 increases ALG-2 levels and miRISC formation in alg-1 loss-of-function animals, thereby compensating for ALG-1 loss and rescuing miRNA-related gildan midnight defects observed.DPF-3 can cleave an ALG-2 N-terminal peptide in vitro but does not appear to rely on this catalytic activity to regulate ALG-2 in vivo.This study uncovers the importance of DPF-3 in the miRNA pathway and provides insights into how multiple miRNA Argonautes contribute to achieving proper miRNA-mediated gene regulation in animals.